Chromatographic studies of the purity of cetokine-like peptides using the alloferon subsstance as an example

Abstract

Alloferon 3 is a natural peptide. It mediates NF-B signaling to enhance recognition of viral, hormonal antigens. It induces the production of endogenous interferons, which contribute to the expression of the cascade of protective reactions, strengthen the CD25 receptor. Cytotoxic lymphocytes are stimulated by alloferons after recognition of non-self or aberrant cells. It is possible to predict the processes of intramolecular interaction, the formation of aqueous substances in which free hydroxy and amino groups are present in the pharmaceutical compositions of alloferon. In the process of synthesis and development of the substance, side products of reactions and impurities are felt, the presence of which affects the quality of the substance. In the process of synthesis and development of a substance, side products of reactions, impurities are formed, the presence of which affects the quality of the substance. An important expansion of the range of instrumental methods for the analysis of the alloferon substance, in addition to those methods recommended by the Pharmacopoeia. Therefore, an urgent task is the adaptation and modification of chromatographic conditions during HPLC analysis, which will allow detection of undeclared APIs in the composition of the substance.