The Role Of Human Papilloma Virus In The Development Of Oral Leukoplakia

Abstract

Aim. To detect the Ki-67 proteins, P16INK4a and antigens of human papillomavirus ofhigh oncogenic risk (HPV16) at hyperplasia, dysplasia and squamous cell carcinoma of the oral mucosa. Materials and methods.The biopsy material of the oral mucosa of 82 patients with leukoplakia was studied, including 42 women and 40 men. The average age of patients was 62 year. At carrying out histological examination unmodified epithelia, which were taken from the adjacent to leukoplakia areas ofmucous membrane, were used as control. As a result of study, 10 (12%) areas ofthe unmodified mucous membrane, 35 (43%) biopsy materials without atypia leukoplakia, 16 (20%) biopsy materialsof leukoplakia with dysplasia of varying degrees from SIN 1 to SIN 3 were studied; a squamous cell carcinoma was detected at 21 (25%) patients. Results and discussion.As a result of study, 10 (12%) areas ofthe unmodified mucous membrane, 35 (43%) biopsy materials without atypia leukoplakia, 16 (20%) biopsy materialsof leukoplakia with dysplasia of varying degrees from SIN 1 to SIN 3 were studied; a squamous cell carcinoma was detected at 21 (25%) patients.IHC study of oral mucosa epithelium has revealedfeaturesof protein expression thatcharacterizes the proliferative processes and viral antigens during different morphological manifestations of leukoplakia showing human papilloma virus replication in proliferating cells of stratified squamous epithelium. Conclusions.At various morphological variants of leukoplakia in epithelial cells of the stratified squamous epithelium of the oral mucosa increased proliferative activity of the cells with the expression in the cell nuclei Ki-67 protein and markers, that are directly (HPV16) or indirectly (P16INK4a) associated with human papillomavirus, is revealed.At hyperplasia with hyperkeratosis only protein P16INK4ais revealed, squamous intraepithelial neoplasia with hyperkeratosis and at squamous cell carcinoma p16INK4A and antigens of HPV of a person at high risk (HPV16)are detected. Keywords:leukoplakia, oral mucosa, immunohistochemistry, human papillomavirus 16 type, Ki-67, Р16INK4а