Morphological characteristics of small intestine mucosa in dysbiosis and after its correction by probiotics and enterosorbents

Abstract

This study was aimed at investigating morphological and functional changes in the small intestine mucosal layer of mice with antibiotic-induced dysbiosis and following its correction with probiotics and enterosorbents. The study was carried out on BalB / c line white laboratory mice. Samples of the small intestine, liver, and spleen were taken to be processed for electron microscopy. To determine qualitative and quantitative composition of intestinal lumenal microbiome, animals’ faeces were studied. Bacteria were isolated and identifed by standard methods. This study has experimentally confrmed the ability of antibacterials to induce dysbiotic conditions in animals that are accompanied by signifcant shifts in the composition of normal microflora, manifested with cytodestructive disorders in the small intestinal epithelium. We have demonstrated the property of probiotics and, to a lesser extent, of sorbents to reduce the intensity and extension of cytodestructive disorders in the course of antibiotic-induced dysbiosis and to normalize the body immune responses that accompany the development of dysbiotic conditions.