Comparative Analysis Of Polysaccharides Of Rosa×Damascena Mill. Buds And Flower Petals

Abstract

The aim. The aim of the work was a comparative study of polysaccharides of Rosa×damascena buds and flower petals after obtaining essential oils. Materials and methods. The water distillation technique was used to determine the essential oils (EO) content in buds and flower petals of R.×damascena. The content of water-soluble polysaccharides (WSPS) and pectins (PC) from buds and flower petals of R.×damascena was obtained using the fractionation method after EO were isolated. Free and bound monosaccharides in WSPS from R.×damascena buds and flower petals were determined by gas chromatography-mass spectrometry (GC/MS) method. Agilent 6890N/5973 inert gas chromato-mass spectrometric system (Agilent Technologies, USA) was used for the chromatographic separation with HP-5ms capillary column (30 m×0.25 mm×0.25 μm, Agilent Technologies, USA). The filtrates obtained after WSPS precipitation of buds and flower petals were analyzed by chemical reactions.