Development Of A Method For Determining Ergocalciferol In Dietary Supplements

Abstract

Vitamin D plays an essential role in calcium and phosphorus metabolism, bone mineralization, and the maintenance of normal immune system function. Among its biologically active forms, ergocalciferol (vitamin D₂) is widely used in fortified foods and dietary supplements, particularly in plant-derived products. In recent years, the global consumption of dietary supplements containing vitamin D has increased significantly due to the widespread prevalence of vitamin D deficiency. However, the complex composition of dietary supplements and variability in manufacturing processes may affect the accuracy of labeled vitamin content. Therefore, reliable and validated analytical methods are required to ensure the quality, safety, and regulatory compliance of these products.