The Need For Improvement Of Fungicides Residual Quantities Control Methods In The Conditions Of The Domestic Regulatory Base Harmonization

Abstract

The aim: To develop highly sensitive analytical methods for the determination of the systemic phenylamide class fungicide – Metalaxyl-M residues in watermelons and grapes to reduce the risk of hazardous effects on workers’ and public health. Materials and methods: Conditions for Metalaxyl-M detection by gas-liquid chromatography (GLC) using a chromatographic capillary column SH-Rxi-5ms (length – 30 m, inner diameter – 0.25 mm, layer thickness – 0.25 μm) were determined. The optimal conditions for chromatography of Metalaxyl-M were established: column thermostat temperature – 220°С, evaporator temperature – 260 °С, detector temperature – 280 °С. The retention time under these conditions was 3,384 ± 0.1 minutes. The linear detection range is 0.01 to 0.05 mg / kg. The calibration dependence of the tested substance peak area on its concentration was established and described by the linear regression equation. Results: We found that the most sensitive method for chromatography of Metalaxyl-M is the method of using a capillary column SH-Rxi-5ms on a gas chromatograph Shimadzu Nexis 2030. Conclusions: The developed GC methods correspond to modern requirements, are selective and allow to control the Metalaxyl-M content in the matrices of the studied crops and can be used as a marker of the safety of agricultural products grown with fungicides containing Metalaxyl-M application. We found that the most sensitive method for Metalaxyl-M chromatography detection is the method with usage of a capillary column SH-Rxi-5ms on a gas chromatograph Shimadzu Nexis 2030.