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Назва: The dynamic of the energy metabolism of the cells of white rats skin connective tissue under conditions of the burn injury and hyperglycemia
Автори: Natrus, Larysa
Ryzhko, Irina
Panova, Tetyana
Osadchuk, Yulia
Ключові слова: cells reactions of connective tissue, burn injury, stable uncorrected hyperglycemia, phenotype of macrophages, enzymes of tissue oxidation
Дата публікації: 2018
Видавництво: Медична наука України
Бібліографічний опис: Natrus L.V., Verko N.P., Ryzhko I.N., Panova T.I., Osadchuk Yu.S. The dynamic of the energy metabolism of the cells of white rats skin connective tissue under conditions of the burn injury and hyperglycemia // Медична наука України. 2018. Т. 14, № 1-2. С. 3-10 https://doi.org/10.32345/2664-4738.1-2.2018.01
Короткий огляд (реферат): The aim of this work is to analyze the oxidoreductases activity of neutrophils, macrophages and fibroblasts in the connective tissue of the white rats skin under conditions of local thermal damage when there is a carbohydrate metabolism violation to predict the efficiency of the tissue regeneration during the repair process. Material and methods. There were carried out the histochemical and pathomorphological researches of the skin burn wound healing dynamic (3, 7, 14, 21 days) of the white mature male rats (Wistar line) with the weight of 180-210g without somatic pathology (control group, n=28) and with stable uncorrected hyperglycemia modeled by streptozotocin (n=28). It has been carried out the histochemical study of oxidoreductase enzymes activity on the cryostat sections (10 μm thickness): succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), NADH- dehydrogenase (NADH-DH). The histochemical index of the oxidoreductase enzymes activity was being calculated by the semi-quantitative method. Results. The study of the cells’ quantitative changes in the connective tissue regenerate and the reactions of energy metabolism against the background of hyperglycemia has some features. The consistency of the connective tissue infiltration by the leukocytes of different types during reparative regeneration after burn injury was being happened in a classical scenario with a quantitative predominance of cells in a group of animals without somatic pathology that points to the reduction of blood reactivity under conditions of glycemia. In the dynamics of the healing process the change of the LDH and NADH-DH activity in the macrophages and fibroblasts had a tendency to increase on the 7th and 14th days of the experiment and differed significantly during observation from the parameters in the 3d day (p<0,05). The most exemplary for study of the dynamics of the oxidation-reduction enzymes activity in the cells of the regenerate was the ratio of SDH/LDH. Comparing the data of the control group of animals and under conditions of hyperglycemia the most expressed and multidirectional changes of the oxidation-reduction enzymes’ activity occurred in macrophages in the different phases of the reparative process. Conclusions. We believe that such differences can indirectly indicate the untimely change of the macrophages’ functional phenotype during restorative processes in the tissues. So as the macrophages are the "main conductor" of intercellular relationships it is suggested that this mechanism may be one of the reasons for the ineffective wound healing under diabetes mellitus.
URI (Уніфікований ідентифікатор ресурсу): http://ir.librarynmu.com/handle/123456789/1111
Розташовується у зібраннях:Наукові публікації науково-дослідного інституту експериментальної та клінічної медицини

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