The phospholipid composition of internal membrane of hepatocytes in rats with glutamat-induced steatohepatitis and its correction by cerium dioxide nanoparticles

Abstract

Lipids are main mitochondrial fuels however these molecules can also behave as uncouplers and inhibitors of oxidative phosphorylation. The development of oxidative stress entails not only a malfunction of mitochondria but also the development of the complications among which the main role play hyperglycemic complications and type 2 diabetes. The aim of our work was to investigate the effect of cerium dioxide nanocrystals on the phospholipid content of the internal mitochondrial membrane of hepatocytes under conditions of glutamate-induced steatohepatitis. As a result of our work it was found that in rats with glutamate-induced steatohepatitis, the percentage content of cardiolipin, lysophosphatidylcholine and lysophosphatidyle than olamine were higher compared with intact control. Under the influence of periodic administration of cerium dioxide nanocrystals in rats with monosodium glutamate induced steatohepatitis, the percentage of cardiolipin in the internal mitochondrial membrane was restored to the level of intact control. In rats with glutamate-induced steatohepatitis, periodic administration of cerium dioxide nanoparticles increased the percentage content of the mixture of phosphatidylinositol and phosphatidylserine compared with intact control and to rats with glutamate-induced steatohepatitis without correction. Our data testify to the positive effect of cerium dioxide nanoparticles on the phospholipid content of the internal membrane of mitochondria, which undergoes significant changes in the development of glutamate-induced steatohepatitis.