Вплив експериментальної спінальної травми на тканинну експресію мрнк деяких елементів медіаторних систем спинного мозку

Abstract

Expression of the matrix RNA (mRNA) proteins Gria1—4, Slc18a2, Slc32a1, Dbh, Tph2, Ptf1a in tissue of a lumbo—sacral spinal cord in mature mongrel male rats in 6 weeks after transection of its left half on a ТXI level was investigated using a PCR method in a real time. Low expression of the tryptophan—hydroxylase 2 (Tph2) and dopamine—β—hydroxylase (Dbh) mRNA was noted in tissue of the intact spinal cord. A spinal cord trauma сauses essential lowering of the Tph2 mRNA expression — homolaterally, and of transmembrane carrier of monoamines Slc18a2 — contralaterally, while in the receptor of glutamate Gria3 subunit — bilaterally. Lateralization is not confirmed by immediate comparison of results of contralateral halves of a spinal cord. Lateralized laminectomy without a spinal cord trauma causes significant bilateral raising of the Gria2 expression in a spinal cord tissue. The data obtained do not correlate with the function indices and spasticity changes of posterior extremities. The lateralized changes of the Tph2 mRNA and carrier of Slc18a2 monoamines, аs well as bilateral changes of mRNA expression in subunit receptor of glutamate Gria3, play important role in pathogenesis of the spasticity and incomplete transection of a spinal cord syndrome.