Influence of silicagel containing gold and silver nanoparticles on the biochemical indices of experimentally-induced inflammation of oral mucosa

Abstract

Aim. Experimental study on rat oral mucosa inflammation and on the influence of drugs containing gold and silver nanoparticles upon the biochemical markers of inflammation and disbiosis. Materials and method. The study was conducted on 42 white rats, in which inflammation of the oral mucosa was experimentally induced by application of lipopolysaccharide (LPS). Preliminary applications, on the oral mucosa, of the gel containing gold and silver nanoparticles were made 2 days before experimental inflammation. The activity of the biochemical inflammation indices was determined in a gingiva and cheek mucosa homogenate. Results. Application of lipopolysaccharide LPS on rat oral mucosa increased the elastase activity and MDA content, which indicates inflammation. Application of gels containing Lyzomucoid with nanoparticles of gold and silver reduces the biochemical inflammation markers, but only in the oral mucosa. Under the action of LPS, catalase activity significantly increased in both gingiva and oral mucosa. The gel containing Lyzomucoid preparations and gold and silver nanoparticles significantly increases the activity of catalase and the antioxidant-prooxidant index (IPA) in both tissues, while decreasing oral mucosa disbiosis. Keywords: oral cavity, lipopolysaccharide, nanoparticles, inflammation, anti-oxidants, disbiosis, gels, lyzomucoids