Using realtime polymerase chain reaction for taxonomic and quantitative analysis of multicomponent probiotics that are used in pediatrics

Abstract

The methods for determining the qualitative and quantitative composition of multicomponent probiotics play an important role in their development and inves tigation. Both taxonomic identification and quantitative analysis of such multisymbiosis components can be significantly accelerated with application of mod ern methods based on the detection and identification of DNA sequences by polymerase chain reaction (PCR). One of the main requirements for this method is the purity of the isolated DNA and the absence of polymerase chain reaction inhibitors in its content. In the course of study, we developed a method for using polymerase chain reaction in real time for qualitative and quantitative determination of the species composition in multicomponent probiotic preparations containing representatives of 18 species of genera Bifidobacterium, Lactobacillus, Propionibacterium, Lactococcus, Streptococcus and Acetobacter. This method allows rapid and accurate research of samples in the presence of various inhibitors of polymerase chain reaction. No conflict of interest was declared by the authors.