Silver nanoparticles: Synthesis, effectiveness in treatment of purulentinflammatory diseases of the maxillofacial area, development of dosage forms

Abstract

Water dispersion of 30 nm spherical silver nanoparticles (AgNP) has been synthesized and characterized as biosafe according to the parameters of cytotoxicity, genotoxicity, mutagenicity and biochemical markers. AgNP’ high bactericidal effectiveness in vitro has been revealed against wide spectra of pathogens (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Haemophilus influenzae, Micrococcus, Candida albicans etc.) isolated from the patients of the Department of Maxillofacial Surgery. High antimicrobial and anti-inflammatory effectiveness in vivo of the AgNP substance has been shown on the model of abscesses of submandibular area in comparison with traditional antiseptic Chlorhexidine. Wistar rats have been used for modeling of abscesses of submandibular area by means of subcutaneous injection in the submandibular area of the suspension containing clinical isolate of Staphylococcus aureus and active carbon powder. The composition and technology of different antiseptic dosage forms (gel, ointment and surgical wound irrigation solution) have been developed using water dispersion of AgNP in different concentration.