Possible therapeutic approach to apoptosis correction in nephrotic children

Abstract

Objectives: the objective of this paper was to study the levels of apoptosis controlling factors and nuclear transcriptional factor NF-κB in relation to the value of kidney function impairment; to evaluate possible protective effects of conventional therapy with addition of antioxidant agent tocopherol in pediatric patients with nephrotic syndrome. Background: hypoxia-related disorders play an important role in irreversible kidney tissue damage via activation of certain secondary processes, i.e. apoptosis. Methods: an examination of blood samples of 53 patients (aged 10 to 15 years) with nephrotic syndrome was done. Conventional clinical investigations, immunoblotting were used in this study. Results: we show that nephrotic patients reveal a high level of pro-apoptotic marker Bax, low level of anti-apoptotic factor BcL-xL and anti-apoptotic unit of NF-κB (p65). Teir dependence on level of kidney function impairment was detected. Applied treatment composes conventional scheme and tocopherol shows a restored balance of apoptotsis controlling factor and NF-κB activity. Conclusion: hypoxia-induces disorders in nephrotic children resulted in apoptosis controlling system disturbances can be attenuated by the antioxidants application.