Microbiological substantiation of the ozone oils usage for the treatment of patients with chronic apical periodontitis

Abstract

The efficacy of endodontic treatment of patients with chronic apical periodontitis determines the rational use of antibacterial medicaments. To elimination of the microflora is used various antibacterial drugs. However, they very quickly became insensitive to the conditionally pathogenic root canal microflora. So relevant is the increase of the effectiveness of antibacterial agents. A promising in this regard is a combination of antibacterial medicaments with ozone. Very important is microbiological assessment of effectiveness ozonotherapy of patients with chronic apical periodontitis. Aim. To determine the microbiological comparative antibacterial activity of non-ozonized and ozonized oil "Eugenol" ("VladMiVa", Russia) on the mixed microflora (aerobic type) from root canal teeth of patients with apical periodontitis. Carrying out a comparative assessment of the quality of root canal treatment after mechanical treatment and application of ozone in chronic periodontitis based on microbiological data. Materials and methods. To determine the sensitivity of microorganisms to the medicaments was used modification of the disk-diffusion method. As a test microorganisms were used reference strains and mixed microflora from the root canal teeth of patients with apical periodontitis. Endodontic treatment was performed on 96 patients (98 teeth) with chronic periodontitis. The material for the background microbiological study was taken immediately after opening of the root canal (before processing the canal). Results. Ozonized oil "Eugenol" showed selective antibacterial properties on mixed microflora (aerobic type) and prolonged duration. After a microbiological research of the root canal content, it was found that root canals with chronic periodontitis in 100 % of cases were infected with various microorganisms, including anaerobic ones. In the initial examination, 125 strains of microorganisms were isolated, and after a thorough chemomechanical treatment of root canals, their number was only 35, the percentage of anaerobic microorganisms remained at the same high level and was 40 %. Conclusion. Ozonized oil "Eugenol" showed selective antibacterial properties and prolonged duration. These microbiological results allow usage this medicaments for clinical use in the treatment of chronic apical periodontitis. Using of ozonotherapy for the treatment of chronic periodontitis in 90.7 % of cases we have been disinfected root canals.