Optimisation of bronchoalveolar lavage technique for isolating alveolar macrophages in mice

Abstract

Bronchoalveolar lavage (BAL) is a widely used technique to collect immune cells from the lungs, with alveolar macrophages (AMs) being the most prevalent cells in BAL fluid. AMs are vital for maintaining lung homeostasis and providing immune defence against airborne pathogens. However, in murine models, BAL procedures usually yield low numbers of AMs, thus limiting experimental design, especially when high cell counts are needed.