Immunohistochemical assessment of CD20 expression in rat kidneys 1 hour after administration of Leiurus macroctenus scorpion venom

Abstract

The aim of the work: to assess the degree of CD20 expression in the kidneys of rats 1 hour after the administration of the venom of the scorpion Leiurus macroctenus. Materials and Methods. The study used 10 white male laboratory rats weighing 200 g (±10 g). The venom of scorpions from the Buthidae family, specifically the genus Leiurus and the species Leiurus macroctenus, was administered to rats intramuscularly (0.5 ml of a previously dissolved venom solution in saline; 28.8 μg/ml; LD50 = 0.08 mg/kg). To identify the subpopulation of CD20 cells in kidney tissue, rabbit recombinant primary antibody Anti-CD20 (ab64088, Abcam, USA) was used.