Expression Features Of Human Papilloma Virus Type 16 And Anti-Epstein-Barr Virus In Pleomorphic Adenoma, Surrounding And Intact Salivary Gland

Abstract

The aim is to reveal the expression features of MCA to human papilloma virus type 16 and anti-Epstein-Barr virus in the pleomorphic adenoma, surrounding and intact salivary gland. Materials and methods: It was used surgical and biopsy material from 30 patients, represented by pleomorphic adenomas with surrounding to tumor tissue of the salivary gland and intact tissue of the salivary gland (the distance between the tumor and the intact salivary gland – 10 mm). Immunohistochemical study was performed using mouse monoclonal antibody (MCA) to human papilloma virus type 16 (clone CAMVIR-1, «Diagnostic BioSystems», USA) and anti-Epstein-Barr virus (LMP, clone CS. 1-4, «Dako», Denmark). Visualization was performed, using an EnVisionTM FLEX detection system (Dako, Denmark). Antigen unmasking was carried out in citrate buffer pH 6.0 at 95°C. Primary antibodies were incubated at room temperature for 30 minutes, secondary antibodies – 20 minutes. Sections were counterstained with Gill hematoxylin. We assessed the immunohistochemical reaction by a semi-quantitative method by counting the percentage of positively stained cells in the field of view of a microscope × 400. Microspecimens were studied and photoarchived on an Olympus BX-41 microscope (Japan).