Potential use of bacteriophages for the oral microbiota modulation in periodontal diseases within the context of maxillofacial pathology

Abstract

Aim: To study the susceptibility of isolates obtained from the oral cavities of individuals with inflammatory periodontal diseases and maxillofacial pathology to bacteriophages. Materials and Methods: Biological specimens were obtained from the Dental Medical Center and University Clinic of O.O. Bogomolets National Medical University and the National Specialized Children’s Hospital OKHMATDYT. Biomaterial was collected from the wound surface using a sterile FLmedical transport system (Italy). Clinical isolates used in the studies included Escherichia coli, Klebsiella spp., Pseudomonas spp., and Staphylococcus aureus. We assessed the susceptibility of microorganisms to bacteriophages by administering 0.01 ml of bacteriophage culture to a bacterial culture. A suspension (inoculum) was prepared from a 24-hour culture of microorganisms in sterile saline and then Mueller Hinton agar was inoculated with it. The results were recorded 24 hours post-incubation in a thermostat at 37°C. The study used bacteriophages from the bacterial test cultures Klebsiella pneumoniae DSM30104, Staphylococcus aureus DSM 799, Escherichia coli DSM 1103, and Pseudomonas aeruginosa DSM 50071, provided by NeoProBioCare Ukraine Ltd.