Microbiological researches of rectal suppositories for the treatment of benign diseases of the prostate gland

Abstract

Background and Aim: Prostatitis is one of the most common and difficult to diagnose and treat conditions in urological practice. Nowadays, the most common types of prostatitis are bacterial and infectious, characterized by acute and chronic cyclic flow with phases of exacerbations and remissions, where particular importance is the involvement of microorganisms, fungi, and protozoa in the pathological process. For the treatment and prevention of benign prostatic diseases, we developed the rectal suppositories with indole-3-carbinol and meloxicam. The aim of this work was to conduct microbiological studies of developed suppositories. Methods: To study the antimicrobial action, six experimental compositions were made. As test cultures, reference strains of bacteria and fungi were used, obtained in the Ukrainian Collection of Microorganisms of the Institute of Microbiology and Virology of D.K. Zabolotny (Kyiv, Ukraine): Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Bacillus subtilis ATCC 6633, and Candida albicans ATCC 10231. The study of the specific antimicrobial action of the medicinal product was conducted in vitro by diffusion in nutrient agar in the modification of “wells.” To determine antibacterial activity, Muller-Hinton agar was used, while studying antifungal action used Saburo dextrose agar. Results: Studies have shown that the presence of meloxicam and excipient Montanox 80 in the composition of suppositories does not significantly affect the degree of the antibacterial and antifungal effects. Suppositories with indole-3-carbinol and the combination of indole-3-carbinol and meloxicam have a pronounced antibacterial effect in relation to Gram-positive and Gram-negative bacteria and antifungal action. Conclusion: The conducted microbiological research has established the possibility and feasibility of using developed rectal suppositories with indole-3-carbinol and meloxicam in the treatment of bacterial and infectious prostatitis.