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Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients

Thu, 01 Jan 2026 00:00:00 GMT

Название: Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients Авторы: Petrenko, O.; Badziukh, S.; Korsa, V.; Kolosovych, I.; Tykhomyrov, A. Краткий осмотр (реферат): Plasminogen (Pg) is currently considered a master regulator of wound healing, but the molecular mechanisms of its efficacy in improving impaired closure of chronic skin ulcers in type 2 diabetes patients remain unclear. Here, we investigated wound healing effects of autologous plasma-derived Pg in diabetes patients with chronic foot ulcers and evaluated Pginduced changes in levels of key protein markers related to wound repair. Type 2 diabetes patients with chronic wounds of lower extremities were included in the study and received topical applications of Pg in a dose of 1.0 mg/mL every 2 days during 20 days, in addition to the standard wound management treatment. Patients treated only according to conventional protocol served as a control. Wound closure rates were monitored by digital planimetry of wound areas. Plasminogen supplementary treatment significantly accelerated relative wound closure as compared with diabetes patients from the control group (24 ± 4 days vs 120 ± 17 days, respectively, P < .01). As shown by Western blot, Pg application reduced expression of protein regulators of hypoxia events, angiogenesis, and autophagy such as hypoxia-inducible factor-1α (by 6.3-folds, P < .01), angiostatins (by 2.5-folds, P < .05), and autophagy marker LC3-II/LC3-I (by 8.6-folds, P < .05), while increasing vascular endothelial growth factor level by 1.9-folds (P < .05). Gelatin zymography showed that Pg-supplemented therapy decreased activity of matrix metalloproteinase-9 (MMP-9) by 3.5-folds at the end of treatment period (P < .01). We report here for the first time that topically applied plasma-derived Pg has a pronounced beneficial effect in promoting foot ulcer healing in patients with type 2 diabetes through preventing hypoxia-induced signaling, reducing autophagy flux, diminishing excessive MMP activity, and enhancing angiogenesis.

The state of neutrophilic granulocyte indices in patients with acute infected necrotizing pancreatitis depending on the type of pathogen

Thu, 01 Jan 2026 00:00:00 GMT

Название: The state of neutrophilic granulocyte indices in patients with acute infected necrotizing pancreatitis depending on the type of pathogen Авторы: Stasenko, A.; Dibrova, Y. Краткий осмотр (реферат): Th e aim of the work was to study the state of polymorphonuclear leukocytes (PMN) in patients with acute infected necrotizing pancreatitis (AINP) depending on the type of pathogen.

Splenic hyperkinetic syndrome: a universal driver of cirrhotic and non-cirrhotic portal hypertension

Thu, 01 Jan 2026 00:00:00 GMT

Название: Splenic hyperkinetic syndrome: a universal driver of cirrhotic and non-cirrhotic portal hypertension Авторы: Kozlov, S.; Kolosovych, I.; Yakovenko, N. Краткий осмотр (реферат): Portal hypertension (PH) is attributed to increased intrahepatic resistance. However, the role of splanchnic hyperdynamic circulation, specifically excessive inflow, remains underappreciated.We hypothesized that splenic arterial hyperperfusion acts as a universal hemodynamic driver in PH - regardless of etiology - creating a volume overload that the portal system cannot accommodate. This study aims to evaluate the relations between splenic arterial inflow and portal venous flow in patients with established cirrhotic and non-cirrhotic PH.

Selection of palliative biliary decompression strategy in unresectable pancreatic head cancer based on predicted postoperative outcomes

Thu, 01 Jan 2026 00:00:00 GMT

Название: Selection of palliative biliary decompression strategy in unresectable pancreatic head cancer based on predicted postoperative outcomes Авторы: Bezrodnyi, B.; Kolosovych, I.; Hanol, I.; Sydorenko, R.; Tsyhanok, A.; Chemodanov, P.; Kondratiev, D. Краткий осмотр (реферат): Current recommendations are focused mainly on determining the indications for systemic therapy and consider biliary decompression as a stage of preparation for its implementation. However, existing scales mainly assess the prognosis of survival and tolerability of chemotherapy, but do not allow predicting the immediate results of decompression intervention.