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Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients

Thu, 01 Jan 2026 00:00:00 GMT

Название: Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients Авторы: Petrenko, O.; Badziukh, S.; Korsa, V.; Kolosovych, I.; Tykhomyrov, A. Краткий осмотр (реферат): Plasminogen (Pg) is currently considered a master regulator of wound healing, but the molecular mechanisms of its efficacy in improving impaired closure of chronic skin ulcers in type 2 diabetes patients remain unclear. Here, we investigated wound healing effects of autologous plasma-derived Pg in diabetes patients with chronic foot ulcers and evaluated Pginduced changes in levels of key protein markers related to wound repair. Type 2 diabetes patients with chronic wounds of lower extremities were included in the study and received topical applications of Pg in a dose of 1.0 mg/mL every 2 days during 20 days, in addition to the standard wound management treatment. Patients treated only according to conventional protocol served as a control. Wound closure rates were monitored by digital planimetry of wound areas. Plasminogen supplementary treatment significantly accelerated relative wound closure as compared with diabetes patients from the control group (24 ± 4 days vs 120 ± 17 days, respectively, P < .01). As shown by Western blot, Pg application reduced expression of protein regulators of hypoxia events, angiogenesis, and autophagy such as hypoxia-inducible factor-1α (by 6.3-folds, P < .01), angiostatins (by 2.5-folds, P < .05), and autophagy marker LC3-II/LC3-I (by 8.6-folds, P < .05), while increasing vascular endothelial growth factor level by 1.9-folds (P < .05). Gelatin zymography showed that Pg-supplemented therapy decreased activity of matrix metalloproteinase-9 (MMP-9) by 3.5-folds at the end of treatment period (P < .01). We report here for the first time that topically applied plasma-derived Pg has a pronounced beneficial effect in promoting foot ulcer healing in patients with type 2 diabetes through preventing hypoxia-induced signaling, reducing autophagy flux, diminishing excessive MMP activity, and enhancing angiogenesis.

The state of neutrophilic granulocyte indices in patients with acute infected necrotizing pancreatitis depending on the type of pathogen

Thu, 01 Jan 2026 00:00:00 GMT

Название: The state of neutrophilic granulocyte indices in patients with acute infected necrotizing pancreatitis depending on the type of pathogen Авторы: Stasenko, A.; Dibrova, Y. Краткий осмотр (реферат): Th e aim of the work was to study the state of polymorphonuclear leukocytes (PMN) in patients with acute infected necrotizing pancreatitis (AINP) depending on the type of pathogen.

Selection of palliative biliary decompression strategy in unresectable pancreatic head cancer based on predicted postoperative outcomes

Thu, 01 Jan 2026 00:00:00 GMT

Название: Selection of palliative biliary decompression strategy in unresectable pancreatic head cancer based on predicted postoperative outcomes Авторы: Bezrodnyi, B.; Kolosovych, I.; Hanol, I.; Sydorenko, R.; Tsyhanok, A.; Chemodanov, P.; Kondratiev, D. Краткий осмотр (реферат): Current recommendations are focused mainly on determining the indications for systemic therapy and consider biliary decompression as a stage of preparation for its implementation. However, existing scales mainly assess the prognosis of survival and tolerability of chemotherapy, but do not allow predicting the immediate results of decompression intervention.

Hemodynamic surgery principles in patients with venous trophic ulcers

Thu, 01 Jan 2026 00:00:00 GMT

Название: Hemodynamic surgery principles in patients with venous trophic ulcers Авторы: Kolosovych, I.; Korolova, K. Краткий осмотр (реферат): Venous trophic ulcers of the lower leg, which account for approximately 80% of all lower leg ulcers, are still a relevant surgical problem. Treatment of chronic venous insuffi ciency (CVI) complicated by ulcers requires an individualized approach, as such patients often have a compromised deep venous system, a history of deep vein thrombosis, and clinical signs of postthrombotic syndrome. For such patients, it is often impossible to use stripping or classic ablation techniques, since it is important to maintain adequate venous blood outfl ow from the lower extremities.