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Model of spinal cord lateral hemi-excision at the lower thoracic level for the tasks of reconstructive and experimental neurosurgery

2023-03-17T08:46:35Z

Название: Model of spinal cord lateral hemi-excision at the lower thoracic level for the tasks of reconstructive and experimental neurosurgery Авторы: Medvediev, V.; Abdallah, I.; Draguntsova, N.; Savosko, S.; Vaslovych, V.; Tsymbaliuk, V.; Voitenko, N.

Morphometric study of rat sciatic nerve recovery after three nerve repair techniques: epineural suture, polyethylene glycol hydrogel and fibrin sealant

2023-03-17T13:20:59Z

Название: Morphometric study of rat sciatic nerve recovery after three nerve repair techniques: epineural suture, polyethylene glycol hydrogel and fibrin sealant Авторы: Goncharuk, O.; Savosko, S.; Petriv, T.; Medvediev, V.; Tsymbaliuk, V. Краткий осмотр (реферат): The effectiveness of microsurgical technique has a direct impact on the recovery of the injured peripheral nerve. The aim of our study was to investigate the result of sciatic nerve regeneration in rats after complete neurotomy and after nerve repair techniques including: 1) epineural suture; 2) polyethylene glycol hydrogel (PEG) (DuraSeal); 3) fibrin sealant (Tisseel). The cross-section of distal sciatic nerve was studied at 14th, 30th and 60th days after nerve repair. Morphometry of myelinated nerve fibers in the distal stump of the sciatic nerve was performed. A significant increase in the number of myelinated nerve fibers was found, especially between 14 and 30 days. The density of myelinated nerve fibers in the distal stump at day 60 was significantly higher after using nerve repair technique including PEG and fibrin versus epineural suture (29.2 % and 32.1 % versus 21.5 %, P <0.05), and a higher level of remyelination of nerve fibers observed in the group with PEG. On day 60, complete elimination of PEG and fibrin sealant was not observed, encapsulation was found around the clusters of hydrogel. Thereby, three peripheral nerve repair techniques were equally effective, only with the use of PEG remyelination of nerve fibers was increasing.

Модель відкритої проникної травми спинного мозку з тривалою персистенцією біосумісного стороннього тіла у каналі хребта. Синдром посттравматичної спастичності

2022-12-09T08:50:33Z

Название: Модель відкритої проникної травми спинного мозку з тривалою персистенцією біосумісного стороннього тіла у каналі хребта. Синдром посттравматичної спастичності Авторы: Цимбалюк, В. І.; Медведєв, В. В.; Гридіна, Н. Я.; Сенчик, Ю. Ю.; Татарчук, М. М.; Драгунцова, Н. Г.; Дичко, С. М.; Петрів, Т. І.

Вплив експериментальної спінальної травми на тканинну експресію мрнк деяких елементів медіаторних систем спинного мозку

2022-12-09T08:37:35Z

Название: Вплив експериментальної спінальної травми на тканинну експресію мрнк деяких елементів медіаторних систем спинного мозку Авторы: Цимбалюк, В. І.; Медведєв, В. В.; Васильєва, І. Г.; Козявкін, В. І.; Галанта, О. C.; Цюбко, О. І.; Чопик, Н. Г.; Олексенко, Н. П.; Драгунцова, Н. Г. Краткий осмотр (реферат): Expression of the matrix RNA (mRNA) proteins Gria1—4, Slc18a2, Slc32a1, Dbh, Tph2, Ptf1a in tissue of a lumbo—sacral spinal cord in mature mongrel male rats in 6 weeks after transection of its left half on a ТXI level was investigated using a PCR method in a real time. Low expression of the tryptophan—hydroxylase 2 (Tph2) and dopamine—β—hydroxylase (Dbh) mRNA was noted in tissue of the intact spinal cord. A spinal cord trauma сauses essential lowering of the Tph2 mRNA expression — homolaterally, and of transmembrane carrier of monoamines Slc18a2 — contralaterally, while in the receptor of glutamate Gria3 subunit — bilaterally. Lateralization is not confirmed by immediate comparison of results of contralateral halves of a spinal cord. Lateralized laminectomy without a spinal cord trauma causes significant bilateral raising of the Gria2 expression in a spinal cord tissue. The data obtained do not correlate with the function indices and spasticity changes of posterior extremities. The lateralized changes of the Tph2 mRNA and carrier of Slc18a2 monoamines, аs well as bilateral changes of mRNA expression in subunit receptor of glutamate Gria3, play important role in pathogenesis of the spasticity and incomplete transection of a spinal cord syndrome.