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Bacteriological and biochemical features of peritonitis in perforative pyloroduodenal ulcer

2026-06-23T12:23:59Z

Название: Bacteriological and biochemical features of peritonitis in perforative pyloroduodenal ulcer Авторы: Kolosovych, I.; Hanol, I. Краткий осмотр (реферат): Despite a wide range of available surgical interventions, the optimal strategy for perforated pyloroduodenal ulcers, including cases combined with other complications, remains undetermined. The use of conventional techniques in patients with peritonitis is often associated with purulent-septic complications. The aim of the work was to investigate the pathophysiological features of peritonitis in perforated pyloroduodenal ulcer and their influence on the course of this disease. The study included 158 patients aged 20-73 years, divided into three groups according to the duration of peritonitis: up to 6 h (n = 99), 6-12 h (n = 35), and more than 12 h (n = 24). All patients underwent bacteriological examination, measurement of abdominal exudate pH at different stages of peritonitis, and analysis of clinical and laboratory parameters. The duration of peritonitis was found to be associated with a higher degree of bacterial contamination of the abdominal cavity. Within 60 minutes after perforation, the initially acidic gastric contents in the abdominal cavity become neutralized; at an exudate pH of 7, optimal conditions for pathogenic microflora are established. Thus, the presented clinical, laboratory and bacteriological results indicate that during the first 3-5 days of the early postoperative period, the patient’s body continues to fight the consequences of post-perforation peritonitis.

Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients

2026-06-04T11:41:16Z

Название: Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients Авторы: Petrenko, O.; Badziukh, S.; Korsa, V.; Kolosovych, I.; Tykhomyrov, A. Краткий осмотр (реферат): Plasminogen (Pg) is currently considered a master regulator of wound healing, but the molecular mechanisms of its efficacy in improving impaired closure of chronic skin ulcers in type 2 diabetes patients remain unclear. Here, we investigated wound healing effects of autologous plasma-derived Pg in diabetes patients with chronic foot ulcers and evaluated Pginduced changes in levels of key protein markers related to wound repair. Type 2 diabetes patients with chronic wounds of lower extremities were included in the study and received topical applications of Pg in a dose of 1.0 mg/mL every 2 days during 20 days, in addition to the standard wound management treatment. Patients treated only according to conventional protocol served as a control. Wound closure rates were monitored by digital planimetry of wound areas. Plasminogen supplementary treatment significantly accelerated relative wound closure as compared with diabetes patients from the control group (24 ± 4 days vs 120 ± 17 days, respectively, P < .01). As shown by Western blot, Pg application reduced expression of protein regulators of hypoxia events, angiogenesis, and autophagy such as hypoxia-inducible factor-1α (by 6.3-folds, P < .01), angiostatins (by 2.5-folds, P < .05), and autophagy marker LC3-II/LC3-I (by 8.6-folds, P < .05), while increasing vascular endothelial growth factor level by 1.9-folds (P < .05). Gelatin zymography showed that Pg-supplemented therapy decreased activity of matrix metalloproteinase-9 (MMP-9) by 3.5-folds at the end of treatment period (P < .01). We report here for the first time that topically applied plasma-derived Pg has a pronounced beneficial effect in promoting foot ulcer healing in patients with type 2 diabetes through preventing hypoxia-induced signaling, reducing autophagy flux, diminishing excessive MMP activity, and enhancing angiogenesis.

The state of neutrophilic granulocyte indices in patients with acute infected necrotizing pancreatitis depending on the type of pathogen

2026-06-03T07:55:53Z

Название: The state of neutrophilic granulocyte indices in patients with acute infected necrotizing pancreatitis depending on the type of pathogen Авторы: Stasenko, A.; Dibrova, Y. Краткий осмотр (реферат): Th e aim of the work was to study the state of polymorphonuclear leukocytes (PMN) in patients with acute infected necrotizing pancreatitis (AINP) depending on the type of pathogen.

Splenic hyperkinetic syndrome: a universal driver of cirrhotic and non-cirrhotic portal hypertension

2026-06-03T07:47:09Z

Название: Splenic hyperkinetic syndrome: a universal driver of cirrhotic and non-cirrhotic portal hypertension Авторы: Kozlov, S.; Kolosovych, I.; Yakovenko, N. Краткий осмотр (реферат): Portal hypertension (PH) is attributed to increased intrahepatic resistance. However, the role of splanchnic hyperdynamic circulation, specifically excessive inflow, remains underappreciated.We hypothesized that splenic arterial hyperperfusion acts as a universal hemodynamic driver in PH - regardless of etiology - creating a volume overload that the portal system cannot accommodate. This study aims to evaluate the relations between splenic arterial inflow and portal venous flow in patients with established cirrhotic and non-cirrhotic PH.