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Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients

2026-06-04T11:41:16Z

Название: Topical Application of Autologous Plasma-Derived Plasminogen Accelerates Healing of Chronic Foot Ulcers in Type 2 Diabetes Patients Авторы: Petrenko, O.; Badziukh, S.; Korsa, V.; Kolosovych, I.; Tykhomyrov, A. Краткий осмотр (реферат): Plasminogen (Pg) is currently considered a master regulator of wound healing, but the molecular mechanisms of its efficacy in improving impaired closure of chronic skin ulcers in type 2 diabetes patients remain unclear. Here, we investigated wound healing effects of autologous plasma-derived Pg in diabetes patients with chronic foot ulcers and evaluated Pginduced changes in levels of key protein markers related to wound repair. Type 2 diabetes patients with chronic wounds of lower extremities were included in the study and received topical applications of Pg in a dose of 1.0 mg/mL every 2 days during 20 days, in addition to the standard wound management treatment. Patients treated only according to conventional protocol served as a control. Wound closure rates were monitored by digital planimetry of wound areas. Plasminogen supplementary treatment significantly accelerated relative wound closure as compared with diabetes patients from the control group (24 ± 4 days vs 120 ± 17 days, respectively, P < .01). As shown by Western blot, Pg application reduced expression of protein regulators of hypoxia events, angiogenesis, and autophagy such as hypoxia-inducible factor-1α (by 6.3-folds, P < .01), angiostatins (by 2.5-folds, P < .05), and autophagy marker LC3-II/LC3-I (by 8.6-folds, P < .05), while increasing vascular endothelial growth factor level by 1.9-folds (P < .05). Gelatin zymography showed that Pg-supplemented therapy decreased activity of matrix metalloproteinase-9 (MMP-9) by 3.5-folds at the end of treatment period (P < .01). We report here for the first time that topically applied plasma-derived Pg has a pronounced beneficial effect in promoting foot ulcer healing in patients with type 2 diabetes through preventing hypoxia-induced signaling, reducing autophagy flux, diminishing excessive MMP activity, and enhancing angiogenesis.

Удосконалення методів профілактики та лікування післяопераційного гіпоспленізму при травматичних ушкодженнях селезінки

2025-10-01T10:53:06Z

Название: Удосконалення методів профілактики та лікування післяопераційного гіпоспленізму при травматичних ушкодженнях селезінки Авторы: Колосович, І. В.; Ганоль, І. В. Краткий осмотр (реферат): З розвитком вторгнення росії в Україну та широкомасштабного застосування нею вогнепальної зброї переважно вибухового характера, виникла велика кількість ран та поліструктурних дефектів тканин, ліквідація яких вимагає етапних складних оперативних втручань повторних хірургічних обробок з ВАК–терапією, закриття отриманих ранових дефектів шляхом дерматопластики, ангіосомними шкірно–м’язовими клаптями та ін. Реабілітація їх довоготривала, з розвитком часткової або повної втрати працездатності(боєздатності),а часто – інвалідизації. Тому пошук найдієвої терапії залишається досі актуальною проблемою.

Choice Of Tactics For Palliative Surgical Treatment Of Patients With Unresectable Pancreatic Head Cancer Complicated By Obstructive Jaundice In Cases Of High Surgical And Anesthetic Risk

2025-08-21T07:17:23Z

Название: Choice Of Tactics For Palliative Surgical Treatment Of Patients With Unresectable Pancreatic Head Cancer Complicated By Obstructive Jaundice In Cases Of High Surgical And Anesthetic Risk Авторы: Bezrodnyi, B.; Kolosovych, I.; Hanol, I.; Slobodianyk, V.; Cherepenko, I.; Chemodanov, P.; Nesteruk, Y. Краткий осмотр (реферат): The disadvantages of open operations to eliminate biliary obstruction and duodenal obstruction in patients with unresectable pancreatic head cancer are a high incidence of postoperative complications (up to 40%) and mortality (up to 15‒20%). When installing self-expanding metal stents (SEMS), the incidence of complications and mortality varies within 1‒3%. However, during surgical bypass operations, effective drainage of the biliary system and stomach lasts for more than a year, and SEMS are subject to obstruction with the development of cholangitis 6 months after installation.

A clinical case of surgical treatment of complicated chronic venous insufficiency using the principles of hemodynamic management

2025-08-21T07:12:15Z

Название: A clinical case of surgical treatment of complicated chronic venous insufficiency using the principles of hemodynamic management Авторы: Kolosovych, I.; Korolova, K. Краткий осмотр (реферат): Chronic venous disease is a widespread condition that involves telangiectasias, reticular veins, varicose veins, and venous ulcers. In addition to the well-known manifestations and complications, long-term persistent chronic venous insufficiency (CVI) can become a factor that contributes to the development of infectious processes in the skin and subcutaneous fat. If the inflammatory lesion spreads deeper, phlegmon and deep vein thrombosis may occur. 20 % to 50 % of patients suffering from deep vein thrombosis develop post-thrombotic syndrome.